Sequencing human rhinoviruses: Direct sequencing versus plasmid cloning
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چکیده
منابع مشابه
Partial Cloning and Nucleotide Sequencing of Glutamate Decarboxylase Gene Isoform 65 from Human Brain
Background: Gamma -aminobutyric acid (GABA), a non-protein amino acid acts as an inhibitory neurotransmitter in the central nervous system of mammalians. The glutamate decarboxylase (GAD) is responsible for the conversion of L-glutamate to GABA. The human brain has two isoforms of this enzyme, GAD65 and GAD67 that differ in molecular weight, amino acid sequence, antigenicity, cellular location ...
متن کاملDirect neutralization of alkaline-denatured plasmid DNA in sequencing protocol by the sequencing reagent itself.
Denaturation of the double stranded DNA is required before sequencing by the dideoxy chain termination method (1). Good results are routinely obtained with the alkaline-denaturation method, which involves incubation of the DNA in the presence of 0.2 M NaOH for a few minutes at room or higher temperatures (2,3). The alkaline mixture is then neutralized by the addition of sodium acetate (0.3 M), ...
متن کاملDirect colony sequencing of plasmid DNA by dye terminator methods.
Direct colony sequencing of plasmid DNA is a powerful technique. Radioactive or fluorescent dye primer methods are the reported direct colony sequencing methods (2–4,6). Recently, dye terminator sequencing chemistry (7) has been improved by the introduction of a modified enzyme, AmpliTaq DNA polymerase FS (8), as well as a new d-Rhodamine and BigDye terminator (PE Biosystems, Foster City, CA,...
متن کاملIDENTIFICATION, ISOLATION, CLONING AND SEQUENCING APARTIALANNEXIN GENE FROM AUREOBASIDIUM PULLULANS
Background and Objectives: Annexin is the common name for genes and proteins that were identified as calcium-dependent phospholipid-binding proteins. Recently a more complex set of functions has been recognized for this superfamily of proteins including in vesicle trafficking, cell division, apoptosis, calcium signalling, mineralization, crystal nucleation inside the extracellular organelle...
متن کاملCloning and Sequencing
The nuclear gene MIPl is strictly required for mitochondrial DNA replication and mitochondrial DNA polymerase activity (Genga, A., Bianchi, L., and Foury, F. (1986) J. Biol. Chem. 261,9328-9332). The MIPl gene was cloned by genetic complementation of the mipl-1 allele after cell transformation with a yeast genomic library and was mapped to the right arm of chromosome XV about 20 centimorgans d...
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ژورنال
عنوان ژورنال: Journal of Virological Methods
سال: 2015
ISSN: 0166-0934
DOI: 10.1016/j.jviromet.2014.09.020